期刊名称：Chemical Industry and Chemical Engineering Quarterly
印刷版ISSN：1451-9372
出版年度：2012
卷号：18
期号：3
页码：431-439
DOI：10.2298/CICEQ110823020K
出版社：Association of the Chemical Engineers
摘要：In this work, removal of dissolved oxygen in water through reduction by glucose, which was catalyzed by glucose oxidase – catalase enzyme, was studied. Central composite design (CCD) technique was applied to achieve optimum conditions for dissolved oxygen scavenging. Linear, square and interactions between effective parameters were obtained to develop a second order polynomial equation. The adequacy of the obtained model was evaluated by the residual plots, probability-value, coefficient of determination, and Fisher’s variance ratio test. Optimum conditions for activity of two enzymes in water deoxygenation were obtained as follows: pH=5.6, T=40°C, initial substrate concentration [S] = 65.5 mmol/L and glucose oxidase activity [E] = 252 U/Lat excess amount of catalase. The deoxygenation process during 30 seconds, in the optimal conditions, was predicted 98.2%. Practical deoxygenation in the predicted conditions was achieved to be 95.20% which was close to the model prediction.
