期刊名称：Journal of Environmental Health Science and Engineering
印刷版ISSN：2052-336X
出版年度：2020
卷号：18
期号：2
页码：1437-1450
DOI：10.1007/s40201-020-00559-9
出版社：BioMed Central
摘要：Abstract Purpose to perform comparative analyzes of the size distributions of bacteria and fungi in the air of overground therapy chambers in Szczawnica sanatorium and subterranean inhalation chambers in Bochnia Salt Mine health resort taking into account influence of the season and presence of pathogenic species. Methods bioaerosol samples were collected using 6-stage Andersen impactor. Bacterial and fungal aerosol concentrations and size distributions were calculated and isolated microorganisms were taxonomically identified based on their morphological, biochemical, and molecular features. Results: in both treatment rooms and atmospheric (outdoor) air, the acceptable microbial pollution levels were periodically exceeded. The size distribution analyzes revealed that in the case of bacteria – emission from the patients and in the case of fungi – transport with atmospheric (outdoor) air were the major processes responsible for microbiological contamination of indoor premises. The majority of microbial particulates were present in the air of studied premises as single bacterial vegetative cells, spores and fungal conidia or (most commonly) formed small microbial or microbial-dust aggregates. This phenomenon may have a significant effect on patients’ actual exposure (especially on those treated for respiratory diseases) in terms of the dose of inhaled particles. Conclusions the microbiological quality of the air in sanatoriums and health resorts is a key factor for their therapeutic and prophylactic functions. When microbial pollution crossed the acceptable level, the measures that enable reducing undesirable contamination should be introduced, especially if large groups of patients undergo such therapy.
其他摘要：Abstract Purpose to perform comparative analyzes of the size distributions of bacteria and fungi in the air of overground therapy chambers in Szczawnica sanatorium and subterranean inhalation chambers in Bochnia Salt Mine health resort taking into account influence of the season and presence of pathogenic species. Methods bioaerosol samples were collected using 6-stage Andersen impactor. Bacterial and fungal aerosol concentrations and size distributions were calculated and isolated microorganisms were taxonomically identified based on their morphological, biochemical, and molecular features. Results: in both treatment rooms and atmospheric (outdoor) air, the acceptable microbial pollution levels were periodically exceeded. The size distribution analyzes revealed that in the case of bacteria – emission from the patients and in the case of fungi – transport with atmospheric (outdoor) air were the major processes responsible for microbiological contamination of indoor premises. The majority of microbial particulates were present in the air of studied premises as single bacterial vegetative cells, spores and fungal conidia or (most commonly) formed small microbial or microbial-dust aggregates. This phenomenon may have a significant effect on patients’ actual exposure (especially on those treated for respiratory diseases) in terms of the dose of inhaled particles. Conclusions the microbiological quality of the air in sanatoriums and health resorts is a key factor for their therapeutic and prophylactic functions. When microbial pollution crossed the acceptable level, the measures that enable reducing undesirable contamination should be introduced, especially if large groups of patients undergo such therapy.
关键词：Air quality;Bioaerosol;Heath resort;Inhalation therapy;Sanatorium;Size distribution
