摘要：The pathogenesis of bovine mastitis caused by Staphylococcus aureus and the presence of S. aureus virulence genes in bovine mastitis are poorly understood. In this study, the virulence factor profiles from different genotyped clinical bovine mastitis S. aureus isolates from The Netherlands were investigated. A total of 76 S. aureus isolates from 2 institutions that represented regional and national strains were examined by PCR for 19 toxin genes, 12 adhesin genes, 3 immune evasion genes and 6 other genes. The presence of hlb , ebps , efb , sspA and sspB was observed in all isolates, coa in all isolates but one, while the presence of sec3, seg, seh, sei, sel, sem, sen, tst , lukE, fnbA , fnbB , i caB , icaC , icaD , clfA, sdrE , cna, cap8 , cap5 and map was variable. Sak and the genes for the human specific immune evasion proteins, SCIN and CHIPS were detected in only one isolate. In addition, none of isolates were lukPV -positive. Five major virulence factor profiles were observed which, with the exception of SaPIbov, were in general agreement with the clustering obtained from PFGE, MLST, spa -typing and MLVA. In conclusion, hlb , ebps , efb , sspA and sspB were present in all isolates and other virulence genes were variably present. The virulence factor profiles generally matched genotyping results for Dutch bovine mastitis isolates.
