摘要:Brucellosis is being reported with exceeding frequency in Iran. Serum antibodies in high-risk and general populations help to determine cut-off points and could be used as simple and fast diagnostic tests in involved areas. We have conducted the serum agglutination test, Combs` Wright and 2-mercaptoethanol titer determination on 908 healthy people. The analysis of our data shown that 275 out of healthy subjects, 30.3% were serum agglutination test and Combs` Wright test positive that 12% of them had titer more than 1:80 and 82.2% had titer less than 1:80. 56.3% of them were 2 mercaptoethanol titer positive. Basic and medium titers in whole of population were between 1:20-1:40. There are no high titers of these antibodies in endemic areas of Iran.