标题：Evaluation of Support Materials for Immobilization of Pycnoporus sanguineus Mycelia for Laccase Production and Biodegradation of Polycyclic Aromatic Hydrocarbons
摘要：The ability of white rot fungus Pycnoporus sanguineus to colonize support materials and subsequently produce laccase and degradation of Polycyclic Aromatic Hydrocarbons (PAHs) was compared with free mycelia culture. Natural support, Ecomat, was found to be the best support material for P. sanguineus for mycelial colonization and laccase activity with maximum activity of 39 nkat mL-1 on day nine of incubation. Coconut husk and grey scouring sponge produces maximum laccase activity of 9.17 and 6.67 nkat mL-1, respectively on day 15. Pycnoporus sanguineus immobilized culture exhibited higher PAHs degradation efficiency compared to the free mycelia culture during the 20 days of incubation. The immobilized mycelia culture degraded 88% of phenanthrene, 93% of anthracene and 85% of pyrene within 20 days. The good correlation between the amount of PAHs degraded and laccase activity produced in the immobilization medium indicated that laccase was solely responsible for degradation of the three PAHs tested. In comparison, free mycelia culture rapidly degraded 42% of phenanthrene, 92% of anthracene and 87% of pyrene at the cessation of incubation. However, poor correlation between the amount of PAHs degraded and laccase activity measured in the free cell culture was obtained. This suggested that intracellular enzymes could be involved in PAHs degradation in the free cell culture.
