摘要：Microbes from diverse and extreme environments are considered to be important sources of enzymes. It is essential that those organisms be provided with optimal growth conditions to increase enzyme production. Effects of pH, temperature, mineral salts, incubation time, sources and concentrations of carbon and nitrogen were tested in submerged fermentation process in production of alkaline protease by Streptomyces sp., isolated from coastal mangrove soil. The production medium prepared in distilled water, supplemented with 5% sucrose ( carbon source ), 7.5% casein (nitrogen source), maintained with pH of 8.5 and incubated at 30°C for 144 h was found optimal for the production of alkaline protease.
