摘要：Flow cytometry (FCM) is widely used in health research and in treatment for a variety of tasks, such as in the diagnosis and monitoring of leukemia and lymphoma patients, providing the counts of helper-T lymphocytes needed
to monitor the course and treatment of HIV infection, the evaluation of peripheral blood hematopoietic stem cell
grafts, and many other diseases. In practice, FCM data analysis is performed manually, a process that requires an
inordinate amount of time and is error-prone, nonreproducible, nonstandardized, and not open for re-evaluation,
making it the most limiting aspect of this technology. This paper reviews state-of-the-art FCM data analysis
approaches using a framework introduced to report each of the components in a data analysis pipeline. Current
challenges and possible future directions in developing fully automated FCM data analysis tools are also outlined.
