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  • 标题:Multicriteria Gene Screening for Analysis of Differential Expression with DNA Microarrays
  • 本地全文:下载
  • 作者:Alfred O. Hero ; Gilles Fleury ; Alan J. Mears
  • 期刊名称:EURASIP Journal on Advances in Signal Processing
  • 印刷版ISSN:1687-6172
  • 电子版ISSN:1687-6180
  • 出版年度:2004
  • 卷号:2004
  • 期号:1
  • 页码:43-52
  • DOI:10.1155/S1110865704310036
  • 出版社:Hindawi Publishing Corporation
  • 摘要:

    This paper introduces a statistical methodology for the identification of differentially expressed genes in DNA microarray experiments based on multiple criteria. These criteria are false discovery rate (FDR), variance-normalized differential expression levels (paired t statistics), and minimum acceptable difference (MAD). The methodology also provides a set of simultaneous FDR confidence intervals on the true expression differences. The analysis can be implemented as a two-stage algorithm in which there is an initial screen that controls only FDR, which is then followed by a second screen which controls both FDR and MAD. It can also be implemented by computing and thresholding the set of FDR P values for each gene that satisfies the MAD criterion. We illustrate the procedure to identify differentially expressed genes from a wild type versus knockout comparison of microarray data.

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