首页    期刊浏览 2024年12月01日 星期日
登录注册

文章基本信息

  • 标题:Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophila neuromuscular junction
  • 本地全文:下载
  • 作者:Sara Seabrooke ; Xinping Qiu ; Bryan A Stewart
  • 期刊名称:BMC Neuroscience
  • 印刷版ISSN:1471-2202
  • 电子版ISSN:1471-2202
  • 出版年度:2010
  • 卷号:11
  • 期号:1
  • 页码:1
  • DOI:10.1186/1471-2202-11-37
  • 语种:English
  • 出版社:BioMed Central
  • 摘要:Background Although the mechanistic details of the vesicle transport process from the cell body to the nerve terminal are well described, the mechanisms underlying vesicle traffic within nerve terminal boutons is relatively unknown. The actin cytoskeleton has been implicated but exactly how actin or actin-binding proteins participate in vesicle movement is not clear. Results In the present study we have identified Nonmuscle Myosin II as a candidate molecule important for synaptic vesicle traffic within Drosophila larval neuromuscular boutons. Nonmuscle Myosin II was found to be localized at the Drosophila larval neuromuscular junction; genetics and pharmacology combined with the time-lapse imaging technique FRAP were used to reveal a contribution of Nonmuscle Myosin II to synaptic vesicle movement. FRAP analysis showed that vesicle dynamics were highly dependent on the expression level of Nonmuscle Myosin II. Conclusion Our results provide evidence that Nonmuscle Myosin II is present presynaptically, is important for synaptic vesicle mobility and suggests a role for Nonmuscle Myosin II in shuttling vesicles at the Drosophila neuromuscular junction. This work begins to reveal the process by which synaptic vesicles traverse within the bouton.
国家哲学社会科学文献中心版权所有