摘要：Diarrhoea caused by opportunistic protozoa is one of the commonest complications seen in the course of HIV disease and is a cause of considerable morbidity and mortality. The present prospective study was conducted on 64 HIV seropositive individuals with diarrhoea to identify the parasitic agents with special reference to the detection of Microsporidia in stool samples and compare the parasite yield with CD4+T cell counts. Apart from routine stool microscopy, modified Ziehl Neelsen and modified trichrome stains were used. Formalin Ethyl Acetate (FEA) sedimentation was the method employed to concentrate the stool parasites. Isospora was the commonest parasite identified (18.75%) followed by Microsporidia (17.18%). Concentration of stool samples by FEA sedimentation was not found to be useful for concentration of Isospora and Microsporidial spores. Maximum parasite yield was seen in patients with CD 4+T cell counts between 50 and 200 cell/µl.
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