出版社：American Society for Biochemistry and Molecular Biology
摘要：Endotoxins (lipopolysaccharides) are the main components ofGram-negative bacterial outer membranes. A quick and simpleway to isolate their lipid region (lipid A) directly from wholebacterial cells was devised. This method using hot ammonium-isobutyratesolvent was applied to small quantities of cells and provedto be indispensable when a rapid characterization of lipid Astructure by mass spectrometry was required. Biological activitiesof endotoxins are directly related to the lipid A structures,which vary greatly with cell growth conditions.
This method is suitable for rough- and smooth-type bacteriaand very efficient for screening variations in lipid A structures.Data are acquired in a few hours and avoid the use of phenolin extraction.Abbreviations: Kdo, 2-keto-3-deoxyoctonate; MALDI, matrix-assisted laser desorption/ionization; LPS, lipopolysaccharide; OS, oligosaccharide; PS, polysaccharide
Supplementary key words endotoxin • lipopolysaccharide • hydrolysis
