出版社：American Society for Biochemistry and Molecular Biology
摘要：The development of a new mass spectrometric lipid profilingmethodology permits the identification of cellular phosphatidylinositolmonophosphate/phosphatidylinositol bisphosphate/phosphatidylinositoltrisphosphate (PIP/PIP2/PIP3) species that includes the fattyacyl composition. Using electrospray ionization mass spectrometry,we were able to resolve and identify 28 PIP and PIP2 compoundsas well as 8 PIP3 compounds from RAW 264.7 or primary murinemacrophage cell extracts. Analysis of PIP profiles after agoniststimulation of cells revealed the generation of differentialPIP3 species and permitted us to propose a novel means for regulationand specificity in signaling through PIP3.
This is the first reported identification of intact, cellularPIP3 by mass spectral analysis. The ability to analyze the fattyacyl chain composition of signaling lipids initiates new venuesfor investigation of the processes by which specific polyphosphoinositidespecies mediate.Abbreviations: AfCS, Alliance for Cellular Signaling; LPA, lysophosphatidic acid; MCF, macrophage colony-stimulating factor; PIP, phosphatidylinositol monophosphate; PIP2, phosphatidylinositol bisphosphate; PIP3, phosphatidylinositol trisphosphate
Supplementary key words lipidomics • phosphoinositides • electrospray ionization mass spectrometry
