出版社：American Society for Biochemistry and Molecular Biology
摘要：Side chain oxidized oxysterols have a unique ability to traverselipophilic membranes. We tested the hypothesis that there isa net flux of 27-hydroxycholesterol from the circulation intothe brain using plasma samples collected from the internal jugularvein and an artery of healthy male volunteers. Two independentstudies were performed, one in which total levels of 27-hydroxycholesterolwere measured and one in which the free fraction of 27-hydroxycholesterolwas measured. In the majority of subjects studied, the levelof 27-hydroxycholesterol was higher in the artery than in the vein,and uptake from the circulation was calculated to be about 5mg/24 h.
The distribution of 27-hydroxycholesterol in human brain wasfound to be consistent with an extracerebral origin, with aconcentration gradient from the white to the gray matterSupplementary key words blood–brain barrier • brain cholesterol • oxysterol • 24S-hydroxycholesterol
