出版社：American Society for Biochemistry and Molecular Biology
摘要：Electrospray ionization mass spectrometry has previously beenused to probe qualitative changes in the phospholipid cardiolipin(CL), but it has rarely been used in a quantitative manner.We assessed changes in the amount of individual molecular speciesof cardiac CL present in a model of congestive heart failureusing 1,1',2,2'-tetramyristoyl cardiolipin as an internal standard.There was a linear relationship between the ratio of the negativemolecular ion ([M-H]–) current from four different CLreference standards and the [M-H]– from the internal standard,as a function of the concentration of CL molecular species.Therefore, this internal standard can be used to quantitatemany naturally occurring CL molecular species over a wide rangeof CL concentrations. Using this method, changes to individualmolecular species of CL in failing hearts from male spontaneouslyhypertensive heart failure rats were examined. CL isolated fromcardiac mitochondria was compared with left ventricular tissueto demonstrate the feasibility of extracting and quantitatingCL from either mitochondrial or tissue samples.
The acyl chain composition of individual CL molecular specieswas identified using tandem mass spectrometry. In animals withheart failure, the major cardiac CL species (tetralinoloyl)decreased significantly, whereas other minor CL species weresignificantly increased.Abbreviations: CL, cardiolipin; ESI, electrospray ionization; LC-MS, liquid chromatography-mass spectrometry; [M-2H]2–, doubly charged negative molecular ion; [M-H]–, negative molecular ion; M4CL, 1,1', 2,2'-tetramyristoyl cardiolipin; MS/MS, tandem collisional mass spectrometry; O4CL, cardiolipin with four oleates; SHHF, spontaneously hypertensive heart failure
Supplementary key words mitochondria • cardiac • internal standard • tandem mass spectrometry
