出版社：American Society for Biochemistry and Molecular Biology
摘要：Our objective was to evaluate the associations of individualapolipoprotein A-I (apoA-I)-containing HDL subpopulation levelswith ABCA1- and scavenger receptor class B type I (SR-BI)-mediatedcellular cholesterol efflux. HDL subpopulations were measuredby nondenaturing two-dimensional gel electrophoresis from 105male subjects selected with various levels of apoA-I in preß-1,-1, and -3 HDL particles. ApoB-containing lipoprotein-depletedserum was incubated with [3H]cholesterol-labeled cells to measureefflux. The difference in efflux between control and ABCA1-upregulatedJ774 macrophages was taken as a measure of ABCA1-mediated efflux.SR-BI-mediated efflux was determined using cholesterol-labeledFu5AH hepatoma cells. Fractional efflux values obtained fromthese two cell systems were correlated with the levels of individualHDL subpopulations. A multivariate analysis showed that twoHDL subspecies correlated significantly with ABCA1-mediatedefflux: small, lipid-poor preß-1 particles (P = 0.0022)and intermediate-sized -2 particles (P = 0.0477). With regardto SR-BI-mediated efflux, multivariate analysis revealed significantcorrelations with -2 (P = 0.0004), -1 (P = 0.0030), preß-1(P = 0.0056), and -3 (P = 0.0127) HDL particles.
These data demonstrate that the small, lipid-poor preß-1HDL has the strongest association with ABCA1-mediated cholesteroleven in the presence of all other HDL subpopulations. Cholesterolefflux via the SR-BI pathway is associated with several HDLsubpopulations with different apolipoprotein composition, lipidcontent, and size.Supplementary key words apolipoprotein A-I • reverse cholesterol transport • ATP binding cassette transporter A1 • scavenger receptor class B type I • high density lipoprotein
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