出版社：American Society for Biochemistry and Molecular Biology
摘要：Acyl-CoA-dependent O-acyltransferases catalyze reactions inwhich fatty acyl-CoAs are joined to acyl acceptors containingfree hydroxyl groups to produce neutral lipids. In this report,we characterize a human multifunctional O-acyltransferase (designatedMFAT) that belongs to the acyl-CoA:diacylglycerol acyltransferase2/acyl-CoA:monoacylglycerol acyltransferase (MGAT) gene familyand is highly expressed in the skin. Membranes of insect cellsand homogenates of mammalian cells overexpressing MFAT exhibitedsignificantly increased MGAT, acyl-CoA:fatty acyl alcohol acyltransferase(wax synthase), and acyl-CoA:retinol acyltransferase (ARAT)activities, which catalyze the synthesis of diacylglycerols,wax monoesters, and retinyl esters, respectively. Furthermore,when provided with the appropriate substrates, intact mammaliancells overexpressing MFAT accumulated more waxes and retinylesters than control cells.
We conclude that MFAT is a multifunctional acyltransferase thatlikely plays an important role in lipid metabolism in humanskin.Abbreviations: ARAT, acyl-coenzyme A:retinol acyltransferase; DGAT, acyl-coenzyme A:diacylglycerol acyltransferase; MFAT, multifunctional acyltransferase; MGAT, acyl-coenzyme A:monoacylglycerol acyltransferase; NCBI, National Center for Biotechnology Information
Supplementary key words neutral lipids • diacylglycerol • fatty alcohol • esterification
