出版社：American Society for Biochemistry and Molecular Biology
摘要：Male rat pups at weaning (21 days of age) were subjected toa diet deficient or adequate in n-3 polyunsaturated fatty acids(n-3 PUFAs) for 15 weeks. Performance on tests of locomotoractivity, depression, and aggression was measured in that orderduring the ensuing 3 weeks, after which brain lipid compositionwas determined. In the n-3 PUFA-deprived rats, compared withn-3 PUFA-adequate rats, docosahexaenoic acid (22:6n-3) in brainphospholipid was reduced by 36% and docosapentaenoic acid (22:5n-6)was elevated by 90%, whereas brain phospholipid concentrationswere unchanged. N-3 PUFA-deprived rats had a significantly increased(P = 0.03) score on the Porsolt forced-swim test for depression,and increased blocking time (P = 0.03) and blocking number (P= 0.04) scores (uncorrected for multiple comparisons) on theisolation-induced resident-intruder test for aggression. Largeeffect sizes (d > 0.8) were found on the depression scoreand on the blocking time score of the aggression test. Scoreson the open-field test for locomotor activity did not differsignificantly between groups, and had only small to medium effectsizes. This single-generational n-3 PUFA-deprived rat model,which demonstrated significant changes in brain lipid compositionand in test scores for depression and aggression, may be usefulfor elucidating the contribution of disturbed brain PUFA metabolismto human depression, aggression, and bipolar disorder.Supplementary key words docosahexaenoic • locomotor • rat • activity • behavior • diet
Abbreviations: ARA, arachidonic acid; DHA, docosahexaenoic acid; DPA, docosapentaenoic acid; EPA, eicosapentaenoic acid; FAME, fatty acid methyl ester; LA, linoleic acid; -LNA, -linolenic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; PUFA, polyunsaturated fatty acid; SSRI, selective serotonin reuptake inhibitor; TLC, thin-layer chromatography
-LNA, 
