出版社：American Society for Biochemistry and Molecular Biology
摘要：Two month old C57BL/6 mice were placed on three different diets:1) normal diet (NC; 0.025% cholesterol), 2) hypercholesterolemicWestern-type diet (HC-W; 0.2% cholesterol), and 3) hypercholesterolemicPaigen-type diet (HC-P; 1.25% cholesterol plus 0.5% cholic acid).At 6 months of age, the animals underwent ligation of the leftcarotid artery and were randomly assigned to vehicle (PBS, subcutaneous)or angiotensin II (Ang II; 1.4 mg/kg/day, subcutaneous) treatmentfor 4 weeks. Low density lipoprotein-cholesterol levels weresimilarly increased in both HC diets (NC, 4 ± 3 mg/dl;HC-W, 123 ± 17 mg/dl; HC-P, 160 ± 14 mg/dl). However,the levels of high density lipoprotein-cholesterol (HDL-C) werereduced only in animals fed the HC-P diet (NC, 82 ± 6mg/dl; HC-W, 79 ± 7 mg/dl; HC-P, 58 ± 7 mg/dl).In Ang II-treated mice, carotid artery ligation induced intimalsmooth muscle cell proliferation to a similar extent in NC-and HP-W-fed animals. However, a significantly larger intimalarea developed in ligated vessels from Ang II-treated mice fedthe HC-P diet (3.6-fold higher than in Ang II-treated NC mice).Together, these results show the accelerating effect of mildhypercholesterolemia, reduced HDL-C levels, and Ang II on intimalhyperplasia after carotid artery ligation in mice.Supplementary key words high density lipoprotein • smooth muscle cells • vascular remodeling
