出版社：American Society for Biochemistry and Molecular Biology
摘要：Skin aging can be attributed to photoaging (extrinsic) and chronological(intrinsic) aging. Photoaging and intrinsic aging are inducedby damage to human skin attributable to repeated exposure toultraviolet (UV) irradiation and to the passage of time, respectively.In our previous report, eicosapentaenoic acid (EPA) was foundto inhibit UV-induced matrix metalloproteinase-1 (MMP-1) expressionin human dermal fibroblasts. Therefore, we investigated theeffects of EPA on UV-induced skin damage and intrinsic agingby applying EPA topically to young and aged human skin, respectively.By immunohistochemical analysis and Western blotting, we foundthat topical application of EPA reduced UV-induced epidermalthickening and inhibited collagen decrease induced by UV light.It was also found that EPA attenuated UV-induced MMP-1 and MMP-9expression by inhibiting UV-induced c-Jun phosphorylation, whichis closely related to UV-induced activator protein-1 activation,and by inhibiting JNK and p38 activation. EPA also inhibitedUV-induced cyclooxygenase-2 (COX-2) expression without alteringCOX-1 expression. Moreover, it was found that EPA increasedcollagen and elastic fibers (tropoelastin and fibrillin-1) expressionby increasing transformin growth factor-ß expressionin aged human skin. Together, these results demonstrate thattopical EPA has potential as an anti-skin-aging agent.Supplementary key words polyunsaturated fatty acid • intrinsic aging • photoaging • ultraviolet irradiation
Abbreviations: AA, arachidonic acid; AP-1, activator protein-1; COX-2, cyclooxygenase-2; DHA, docosahexaenoic acid; ECM, extracellular matrix; EPA, eicosapentaenoic acid; MAPK, mitogen-activated protein kinase; MED, minimal erythma dose; MMP, matrix metalloproteinase; PG, prostaglandin; TGF-ß, transforming growth factor-ß; UV, ultraviolet
