出版社：American Society for Biochemistry and Molecular Biology
摘要：The structures of two major phosphoglycolipids from the thermophilicbacteria Thermus oshimai NTU-063, Thermus thermophilus NTU-077,Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220were determined using spectroscopic and chemical analyses tobe 2'-O-(1,2-diacyl-sn-glycero-3-phospho) -3'-O-(-N-acetyl-glucosaminyl)-N-glyceroylalkylamine [PGL1 (1)] and the novel structure 2'-O-(2-acylalkyldio-1-O-phospho)-3'-O-(-N-acetylglucosaminyl)-N-glyceroylalkylamine [PGL2 (2)]. PGL2 (2) is the first phosphoglycolipididentified with a 2-acylalkyldio-1-O-phosphate moiety. The fattyacids of the phosphoglycolipids are mainly iso-C15:0, -C16:0,and -C17:0 and anteiso-C15:0 and -C17:0. The ratios of PGL2(2) to PGL1 (1) are significantly altered when grown at differenttemperatures for three strains, T. thermophilus NTU-077, M.ruber NTU-124, and M. taiwanensis NTU-220, but not for T. oshimaiNTU-063. Accordingly, the ratios of iso- to anteiso-branchedfatty acids increase when grown at the higher temperature.Abbreviations: CE-MS, capillary electrophoresis-mass spectroscopy; DQF-COSY, double quantum filtered-correlation spectroscopy; FAME, fatty acid methyl ester; gHMQC, gradient heteronuclear multiple quantum coherence; HMBC, heteronuclear multiple bond coherence; HPAEC-PAD, high-performance anion-exchange chromatography with pulsed amperometric detection; MALDI, matrix-assisted laser desorption ionization; PGL1, 2'-O-(1,2-diacyl-sn-glycero-3-phospho)-3'-O-(-N-acetyl-glucosaminyl)-N-glyceroyl alkylamine; PGL2, 2'-O-(2-acylalkyldio-1-O-phospho)-3'-O-(-N-acetylglucosaminyl)-N-glyceroyl alkylamine; ROESY, rotational frame nuclear Overhauser effect spectroscopy; TOCSY, total correlation spectroscopy; 2D, two-dimensional
Supplementary key words nuclear magnetic resonance spectroscopy • matrix-assisted laser desorption ionization mass spectroscopy • capillary electrophoresis-mass spectroscopy • tandem mass spectrometry • Thermus oshimai NTU-063 • Thermus thermophilus NTU-077 • Meiothermus ruber NTU-124 • Meiothermus taiwanensis NTU-220
-N-acetyl-glucosaminyl)-N-glyceroyl alkylamine [PGL1 (1)] and the novel structure 2'-O-(2-acylalkyldio-1-O-phospho)-3'-O-(
