出版社：American Society for Biochemistry and Molecular Biology
摘要：Serum levels of total glycerides and free glycerol are importantindices of lipid metabolism and cardiovascular disease risk.Convenient enzymatic methods of measurement have been available,but they are susceptible to interference. Situations exist inboth research and clinical laboratories in which more specificand precise methods are needed. We developed HPLC methods forthe measurement of serum total glycerides and free glycerol.For total glycerides, serum was mixed with an internal standard(1,2,4-butanetriol) and treated with alcoholic sodium hydroxideto hydrolyze glycerides to glycerol. After deproteinizationwith tungstic acid, the glycerol was benzoylated with an optimizedSchotten-Baumann reaction and analyzed by HPLC. For free glycerol,serum was equilibrated with the internal standard and deproteinizedwith tungstic acid to remove the glycerides. The glycerol wasbenzoylated and analyzed as for total glycerol. Various factorswere investigated, and no significant sources of interferencewere detected. The total coefficients of variation ranged from0.7% to 2.0% for total glycerides and from 1.7% to 3.2% forfree glycerol. The analytical recoveries ranged from 98.5% to101.6%. In conclusion, simple and reliable HPLC methods forserum total glycerides and free glycerol have been developed.The methods may also be used for the analyses of glycerol orglycerides in other biological samples.Supplementary key words triglycerides • Schotten-Baumann reaction • benzoylation • butanetriol
