出版社：American Society for Biochemistry and Molecular Biology
摘要：Maternal diabetes can cause fetal macrosomia and increased riskof obesity, diabetes, and cardiovascular disease in adulthoodof the offspring. Although increased transplacental lipid transportcould be involved, the impact of maternal type 1 diabetes onmolecular mechanisms for lipid transport in placenta is largelyunknown. To examine whether maternal type 1 diabetes affectsplacental lipid metabolism, we measured lipids and mRNA expressionof lipase-encoding genes in placentas from women with type 1diabetes (n = 27) and a control group (n = 21). The placentaltriglyceride (TG) concentration and mRNA expression of endotheliallipase (EL) and hormone-sensitive lipase (HSL) were increasedin placentas from women with diabetes. The differences weremore pronounced in women with diabetes and suboptimal metaboliccontrol than in women with diabetes and good metabolic control.Placental mRNA expression of lipoprotein lipase and lysosomallipase were similar in women with diabetes and the control group.Immunohistochemistry showed EL protein in syncytiotrophoblastsfacing the maternal blood and endothelial cells facing the fetalblood in placentas from both normal women and women with diabetes.These results suggest that maternal type 1 diabetes is associatedwith TG accumulation and increased EL and HSL gene expressionin placenta and that optimal metabolic control reduces theseeffects.Supplementary key words free fatty acids • endothelial lipase • hormone sensitive lipase
Abbreviations: EL, endothelial lipase; HbA1c, hemoglobin A1c; HSL, hormone-sensitive lipase; LAL, lysosomal acid lipase; TG, triglyceride
