出版社：American Society for Biochemistry and Molecular Biology
摘要：Cyclooxygenase (COX)-2 plays an important role in brain arachidonicacid (20:4n-6) metabolism, and its expression is upregulatedin animal models of neuroinflammation and excitotoxicity. Ourhypothesis was that brain lipid composition would be alteredin COX-2 knockout (COX-2–/–) compared with wild-type(COX-2+/+) mice, reflecting the important role of COX-2 in brainlipid metabolism. Concentrations of different lipids were measuredin high-energy microwaved brain from COX-2–/– andCOX-2+/+ mice. Compared with the COX-2+/+ mouse brain, the brainof the COX-2–/– mouse had a statistically significant15% increase in phosphatidylserine (PtdSer) and significant37, 27, and 32% reductions in triacylglycerol and cholesterolconcentrations and in the cholesterol-to-phospholipid ratio,respectively. The normalized concentration of palmitic acid(16:0) was increased in PtdSer, as was the brain concentrationof unesterified arachidic acid (20:0). A lifetime absence ofCOX-2 produces multiple changes in brain lipid composition.These changes may be related to reported changes in fatty acidkinetics and in resistance to neuroinflammation and excitotoxicityin the COX-2–/– mouse.Supplementary key words fatty acids • cholesterol • phosphatidylserine
Abbreviations: AA, arachidonic acid; CerPCho, sphingomyelin; ChoGpl, choline glycerophospholipid; COX, cyclooxygenase; cPLA2, cytosolic phospholipase A2; EtnGpl, ethanolamine glycerophospholipid; FAME, fatty acid methyl ester; PtdCho, phosphatidylcholine; PtdEtn, phosphatidylethanolamine; PtdIns, phosphatidylinositol; PtdSer, phosphatidylserine; sPLA2, secretory phospholipase A2
