出版社：American Society for Biochemistry and Molecular Biology
摘要：Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa proteinbelonging to the lipocalin protein superfamily. Although predominantlyassociated with high density lipoprotein, apoM is found in allmajor lipoprotein classes. To facilitate clinical studies ofapoM, we have developed a sandwich ELISA for the measurementof apoM in human plasma. This method has been used to investigatenormal apoM variation and to establish reference values forhealthy individuals through the measurement of 598 samples fromthe Nordic Reference Interval Project Bio-bank and Database(NOBIDA) biobank. For women 18–49 years old, the referenceinterval for apoM was 0.58–1.18 µmol/l, whereasfor women 50+ years and for men, the reference range was 0.61–1.30µmol/l. Correlation studies of apoM with 26 common clinicalchemical analytes from the NOBIDA database revealed a markedpositive correlation with plasma total cholesterol (r = 0.52)and LDL and HDL cholesterol (r = 0.43 and 0.36, respectively).There was no statistically significant correlation with HDL/totalcholesterol ratio or body mass index. In conclusion, a sandwichELISA for the measurement of apoM in human plasma shows thatapoM concentration is strongly correlated to total cholesterolin healthy individuals.Supplementary key words high density lipoprotein • low density lipoprotein • reference interval • reference range • biobank • Nordic Reference Interval Project Bio-bank and Database • Nordic Reference Interval Project • lipocalin • enzyme-linked immunosorbent assay
