期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2007
卷号：14
期号：02
页码：209-209
出版社：Institute of Agricultural Medicine in Lublin
摘要：The aim of the study was to assess the frequency of Borrelia burgdorferi DNA
detection in the blood and urine of patients diagnosed with erythema migrans, and compare
the results of PCR-based methods with ELISA methodology. The latter was used to
detect serum antibodies against Borrelia burgdorferi of the IgM and IgG classes, before
and after antibiotic therapy. The study included 86 patients hospitalized in the Department
of Infectious Diseases and Neuroinfections in the Medical Academy in Białystok, diagnosed
with the erythema migrans phase of Lyme borreliosis. Examinations were carried
out twice: the fi rst at the moment of diagnosis (Trial 1), the second after 4 weeks of antibiotic
therapy. The study showed that antibiotic therapy in the early phase of borreliosis
does not decrease the sensitivity of PCR and that after 4 weeks of therapy (Trial 2), spirochete
DNA is still detectable in most patients (45/86). There was no correlation between
detectability of spirochete DNA and the presence of antibodies against B. burgdorferi s.l.
(assessed by ELISA) during the course of erythema migrans. The largest percentage of
positive results in the detection of B. burgdorferi s.l. DNA was observed in patients who
simultaneously possessed IgM and IgG antibodies against B. burgdorferi, while the lowest
percentage of PCR positive results was among patients with only IgM antibodies.
