期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2007
卷号：14
期号：02
页码：215-215
出版社：Institute of Agricultural Medicine in Lublin
摘要：A duplex-PCR method, with 2 pairs of primers recognizing sequences of mitochondrial
D-loop region, was developed to identify cows’ milk in the milk of goats. The
PCR was shown to be specifi c and sensitive, enabling the detection of less than 1% of
cows’ milk added to the milk of goats. Simultaneous use of a primer pair for goats’ and
cows’ mitochondrial DNA fragment prevented false negative results. The method was
applied to track the presence of cow DNA in goat milk available on the Polish market.
A total of 54 milk samples from 3 Polish (34) and one foreign producer (20) were examined.
In 33 samples, cow DNA was detected, while 21 samples, including all of the 20
samples from foreign producers, produced the goat-specifi c product only
关键词：PCR, species identifi cation, food-allergy, cow milk, goat milk.
