期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2007
卷号：14
期号：02
页码：265-265
出版社：Institute of Agricultural Medicine in Lublin
摘要：Cryptosporidium spp. and Giardia spp. are wide-spread pathogens of humans
and many species of mammals. The ways of transmission are very complex and diffi cult
to defi ne. Both parasites occur in similar environments and share a broad host range.
However, in Poland there is still little known about the epidemiology of these parasites
due to the paucity of data on human cases and only few studies in wildlife. The aim of
our study was to determine the distribution of two intestinal protozoa in a few species of
protected and game mammals in North-Eastern Poland. Additionally, we wanted to compare
prevalence and abundance of these parasites between wild and farm animals, and
to determine the species/genotypes of Cryptosporidium. Fecal samples collected from
protected species (European beaver – 22, grey wolf – 14, European bison – 55, Polish
Konik (horse) – 5) and game mammals (red deer – 52, roe deer – 22, boar – 5) were examined
by IFA. We also studied a group of samples collected from farm animals: beaver – 30,
red deer – 66, Polish konik – 5. Cryptosporidium oocysts were identifi ed in 5 of 7 studied
animal species (prevalence from 9% in roe deer to 36% in wolves), Giardia cysts in 4
of 6 studied species (prevalence from 1.7% in red deer to 7.7% in European beaver).
Sequencing analysis of COWP gene fragment revealed that 5 Cryptosporidium isolates
from wolves were C. parvum genotype 2 (zoonotic). The results show the important role
of examined species in maintaining the natural sources of Cryptosporidium spp. and Giardia
spp. infections in the environment.
