期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2007
卷号：14
期号：02
页码：291-291
出版社：Institute of Agricultural Medicine in Lublin
摘要：Air samples were collected on glass fi bre fi lters in 22 animal houses and 3
hay storage barns and examined for the presence of bacterial endotoxin with the Limulus
(LAL) test and the gas chromatography – tandem mass spectrometry (GC-MSMS)
technique, based on detection of 3-hydroxy fatty acids (3-OH-FAs) as chemical markers
of the endotoxin lipopolysaccharide. The median concentrations of airborne endotoxin
determined with LAL test in poultry houses, sheep sheds, piggeries, cow barns,
and horse stables were respectively 62.49 μg/m3, 26.2 μg/m3, 3.8 μg/m3, 1.65 μg/m3, and
1.14 μg/m3, while those determined with the GC-MSMS technique were respectively 1.06
μg/m3, 7.91 μg/m3, 0.2 μg/m3, 0.31 μg/m3, and 1.42 μg/m3. The median concentrations of
airborne endotoxin determined with LAL test and GC-MSMS technique in hay storage
barns were much smaller, 0.09 μg/m3 and 0.03 μg/m3, respectively. The concentrations of
airborne endotoxin (LPS) detected with GC-MSMS method in the air of sheep sheds were
signifi cantly greater than in all other examined facilities, while those detected in hay storage
barns were signifi cantly smaller than in all other examined facilities (p<0.05). The
concentrations of airborne endotoxin determined with LAL test and GC-MSMS analysis
exceeded in most of animal houses examined (91% by each method) the threshold limit
value for airborne endotoxin of 0.1 μg/m3 proposed by various authors. A signifi cant correlation
(p<0.05) between the concentrations of endotoxin determined with the LAL and
GC-MSMS techniques was found in the air samples collected in poultry houses and sheep
sheds, but not in other examined facilities. 3-OH FAs with C14-C18 chains were predominant
in the air of the facilities under study. A signifi cant correlation (p<0.05) was found
between the concentrations of endotoxin determined with LAL test and the amounts of
3-OH FAs with C14-C16 chains. In conclusion, endotoxin in the concentrations detected in
this study may present a respiratory hazard to both humans and livestock animals.
关键词：endotoxin, organic dust, air, animal houses, gas chromatography, mass
spectrometry, 3-hydroxy fatty acids, Limulus test.
