出版社：German Medical Science GMS Publishing House, Düsseldorf
摘要：Optical non-invasive methods have become more and more important for the characterization of skin lesions and for therapy control. In vivo laser scanning microscopy is a promising method which can be used for the analysis of cellular structures in the skin up to a depth of 250 µm. Therefore, laser scanning microscopy (LSM) is well-suited for the characterization of wound healing processes. In contrast to measurements of the transepidermal waterloss (TEWL) the laser scanning microscopy allows the analysis of the healing process on a cellular level. The course of wound healing determined by laser scanning microscopy was correlated with numerical values, which correspond to those used for measurements of TEWL, allowing the numerical characterization of the wound healing process.
Laser scanning microscopy showed that wound healing starts not only from the wound edges but also from "islands" inside the wound area.
In contrast to TEWL measurements the LSM analysis is not influenced by disturbing factors such as temperature, humidity and topically applied substances. Therefore, the laser scanning microscopy is well-suited for the characterization of different types of wound healing therapies including the topical application of creams and lotions.
