期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2008
卷号：15
期号：01
页码：135-135
出版社：Institute of Agricultural Medicine in Lublin
摘要：A total of 67 samples of tap water were collected from faucets and shower-
heads in 6 hospitals located in the Lublin province (eastern Poland). The samples were
examined for the presence and species composition of Legionella, Gram-negative bac-
teria belonging to family Enterobacteriaceae (GNB-E) and Gram-negative bacteria not
belonging to family Enterobacteriaceae (GNB-NE), by ? ltering through cellulose ? lters
and culture on respectively GVPC, EMB and tryptic soya agar media. On average, Le-
gionella was isolated from 65.7% of the water samples taken in hospitals. Strains of the
Legionella pneumophila types 2¨C14 predominated, forming 74.6% of totalLegionella
isolates. Legionella pneumophila type 1 strains constituted 13.5% of the total count,
while other species of Legionella (referred to as Legionella spp.) formed 11.9% of the
total. The concentrations of Legionella in positive water samples ranged from 3¨C350
cfu/100 ml. GNB-E were not found in the examined water samples. GNB-NE were iso-
lated from 79.1% of the water samples taken in hospitals in the concentrations 11¨C300
cfu/100 ml. Species of the family Pseudomonadaceae predominated among GNB-NE
strains isolated from the examined water samples, forming on average 71.5% of the to-
tal count. Altogether, 20 GNB-NE species were identi? ed in the examined samples, out
of which 12 were potentially pathogenic. In conclusion, Gram-negative ? ora of water
samples taken in the examined hospitals complies with potable water sanitary standards
by the lack of Enterobacteriaceae species, but creates a moderate health risk because of
mediocre concentrations of Legionella and the presence of potentially pathogenic non-
enterobacterial species.
关键词：Gram-negative bacteria, Legionella, Non-Enterobacteriaceae, water
distribution systems, hospitals.
