期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2008
卷号：15
期号：01
页码：167-167
出版社：Institute of Agricultural Medicine in Lublin
摘要：Borrelia burgdorferi sensu lato (s.l.) complex, the etiological factor of Lyme
disease, includes over a dozen species of bacteria and 3 pathogenic within it. According
to many authors, the clinical symptoms of borreliosis depend on the species that cause
the disease. The most frequent symptom of early localized borreliosis is erythema mi-
grans (EM). The aim of the research was to determine species of B. burgdorferi s.l. in 32
patients from the Western Pomerania region in whom EM has been recognized. Blood
samples of patients were investigated by PCR-RFLP method, with the use of enzyme dif-
ferentiating species. The DNA of spirochetes was detected in 25 patients (25/32, 78.1%),
compared with 23/32 (71.8%) of ELISA positive patients. Among 25 positive samples,
10 contained the DNA of B. garinii (10/25, 40%), 5 the DNA of B. afzelii (5/25, 20%), 4
the DNA of B. burgdorferi sensu stricto (s.s.) (4/25, 16%) and in 6 samples (6/25, 24%)
the DNA of both B. garinii and B. afzelii was found. The DNA of B. burgdorferi s.l. spiro-
chetes may be detected in patients with EM after antibiotic treatment. The most frequent
species in patients with EM from the Western Pomerania region is B. garinii. Infections
with more than one species of B. burgdorferi s.l. may occur in patients with EM.
关键词：Lyme disease, erythema migrans, Borrelia burgdorferi, species of
B. burgdorferi s.l., PCR, ELISA serology.
