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  • 标题:DETERMINATION OF TOCOPHEROLS CONTENT IN SUNFLOWER OIL DURING OXIDATION USING FLUORESCENCE TECHNIQUE
  • 本地全文:下载
  • 作者:Paweł Górnaś ; Aleksander Sieger ; Krzysztof Dwiecki
  • 期刊名称:Acta Scientarum Polonorum - Technologia Alimentaria
  • 印刷版ISSN:1644-0730
  • 电子版ISSN:1898-9594
  • 出版年度:2006
  • 卷号:5
  • 期号:2
  • 页码:157-164
  • 出版社:Agricultural University of Poznan Press
  • 摘要:The fluorescence intensity of tocopherols originated from cold-pressed sunflower oil at different degree of oxidation in presence of 1,2-dipalmitylo-sn-glicero-3- -fosfatidylocholine lipid (DPPC) membrane have been measured. It has been shown that fluorescence of tocopherol depends on amount of oil as well as on membrane concentra-tion in the sample. Addition of oil with increasing peroxide value PV decreased the toco-pherol fluorescence due to its disappearance in the sample. At constant membrane con-centration chromatographically determined the amount of tocopherol in oil sample corre-lated with peroxide value and fluorescence intensity. Such results allowed us construct calibration curve which may be used for fast and accurate peroxide value determination using fluorescence technique.
  • 关键词:sunflower oil, tocopherols, peroxide value, DPPC membrane, fluorescence
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