期刊名称：Western Australia. Fisheries Department. Fisheries Research Reports
印刷版ISSN：1035-4549
出版年度：2008
卷号：178
页码：204-204
出版社：Government of Western Australia / Department of Fisheries
摘要：Protein concentrates and isolates were made from lupin kernel flours using standard soybean
industry processes. Lupinus angustifolius kernel flour with starting protein content of 46.1%
crude protein (CP) (N x 6.25) achieved a protein content of 52% CP after washing with a 70%
ethanol solution (10:1) while the L. luteus lupin kernel flour with starting protein content of
52.0% CP achieved a protein content of 71.7% CP. The fraction of the flour which is removed
by washing is predominantly oligosaccharides which make up 8% of the L. angustifolius kernel
flour and 14% of the L. luteus kernel flour. L. luteus flour reached the 65% benchmark set by
the soy industry for protein concentrates. Pre-heat treatment of the lupin kernel flours makes
the lupin proteins less soluble in the aqueous medium allowing for the oligosaccharides to be
removed with water without the need for ethanol. Lupin protein isolates were prepared from
L. angustifolius, L. albus, L. luteus and L. mutabilis) by solubilisation at pH 9, removing the
insoluble residue (fibre) and acid precipitation (pH 4.5). Overall the protein recoveries are similar
(~ 85% to 90%) for all four species with the exception of L. albus, which was approximately
10 per cent lower. L. luteus and L. mutabilis appear to be excellent protein sources for the
production of protein isolates given the high initial kernel protein concentrations. However, a
de-fatting step would have to be introduced for L. mutabilis. Particle size of the kernel flour
influenced the amount of protein lost with the fibre fraction. Extended soaking of the kernel
flour in water at native pH5.5 resulted in significant protein recoveries. Pectinase treatment did
not improve the protein extraction yields. Given the yields and production costs, concentrates
from L. luteus appear to be the most viable option for production of a grain protein concentrate
for use in the aquaculture feed industry.
Loading...
