标题：Effects of the heterocyclic amine 2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline (MeIQx) on human lymphocyte DNA in diabetic and non-diabetic population sub-groups: an assessment using the Comet Assay. Susceptibility in human lymphocytes to MeIQx23
摘要：2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline (MeIQx) is one of the most
abundant heterocyclic amines (HAs) in cooked food, and has been subjected to
numerous toxicological procedures. Our aim was to assess the extent of DNA
damage induced by MeIQx in lymphocytes in the alkaline Comet assay, and to
compare various population sub-groups. Materials and methods . The alkaline
Comet assay was used to investigate lymphocytes from 63 individuals (diabetic
and non-diabetic, different ethnicities, sexes, ages, diets, etc) in vitro. Results . No statistically significant
differences in susceptibility to DNA damage were observed between subject
covariates using linear regression (multivariate analysis) techniques. However, using non-parametric data analysis, statistically significant differences were seen
between groups above and below 45 years of age. Conclusions . MeIQx produced highly
statistically significant, yet relatively low levels of alkali labile sites, and
single strand breaks in DNA in lymphocytes. There were no differences in
susceptibility in population sub-groups to this dietary compound.
