The use of eHealth to enhance healthcare provision is becoming a global objective. eHealth readiness assessment tools can determine the preparedness of healthcare institutions to adopt programs related to eHealth. These tools can become important components of eHealth implementation, especially in developing countries. Objective of this study was to validate eHealth readiness assessment tools for healthcare institutions in developing countries, in public and private healthcare institutions of Pakistan. Qualitative methods, borrowed from the tradition of ‘case-study’, were used to determine the face and content validity of eHealth readiness assessment tools. All healthcare institutions in Pakistan were taken as a single case, and in-depth interviews were conducted with managers and healthcare providers from four institutions. All the participants were involved in planning and implementation of eHealth programs. eHealth readiness assessment tools were considered comprehensive, relevant, and beneficial for each level and sector of care. Participants’ views were obtained on the importance, relevance, and placement of each item and section in the tools. Valuable insight and suggestions for development and improvement of the tools were provided. These suggestions included placing extra emphasis on certain issues based on the conditions in Pakistan, such as comfort with the use of technology in maintaining patients’ records, proper budgeting and identification of sources of funds, external evaluation, socio-cultural factors among staff and clients, and building trust among policy-makers, senior administrators and faculty members. eHealth readiness assessment tools were found valid for face and content validity.