There is a need for high quality evaluation instruments when testing new mobile devices and services. The aim of this study was to describe the development of the MobiHealth Usability Evaluation Questionnaire. It was developed in a step-wise consensus process with close interaction between researchers, technology developers and end-users. The questionnaire ten dimensions: functionality, user interface, effectiveness, efficiency, satisfaction, safety, functional and aspirational needs, mastery and empowerment, mobility and activity, quality of life and ethical considerations. These dimensions are defined and their usage as parameters in an evaluation instrument is discussed. The conclusion is that the MobiHealth Evaluation Questionnaire can be used as a basis for method development when evaluating mobile care and rehabilitative devices, but it should be tested for reliability before actual use.