摘要：Background: Brucellosis is a zoonotic disease and its symptoms
and appearances are not exclusive in human and its traditional diagnosis is
based on blood culture and serological methods. For more sensitive and specific
detection, the PCR method is recommended. Methods: One
hundred four blood samples were gathered from suspicious patients with
brucellosis different ages form Kermanshah, Mazandaran, Khorassan and
Hormozgan provinces, and were examined by Rose Bengal, anti globulin, culture
and PCR methods. Results: Seventy three samples were
positive by PCR method, 15 samples were positive by cultured method and 84
samples were positive by serological
methods. Conclusion: PCR method is sensitive and
specific for diagnosis of Brucella from peripheral blood in suspected
cases
关键词：Brucella ، Culture and serological methods ، PCR
