期刊名称：Boletim Centro de Pesquisa e Processamento de Alimentos
印刷版ISSN：0102-0323
电子版ISSN：1983-9774
出版年度：2004
卷号：22
期号：01
出版社：Centro de Pesquisa e Processamento de Alimentos
摘要：Aiming the development of special dietetic formulations for phenylketonuria, five
enzymatic hydrolysates from skim milk powder were prepared, using papain (PA)
and pepsin (PE), separately or in association with protease from Aspergillus oryzae
(AO). The activated carbon was used to remove phenylalanine (Phe) from
hydrolysates being tested the treatments in Beaker and in column. The treatment in
column by direct passage, in 20 mL syringe, using 90 g of hydrated carbon/g of
casein, was the chosen condition for Phe removal from skim milk powder hydrolysates. The isolated action of PA and PE, and the association of PA with AO
produced the highest Phe removal (97 to 98%). The second derivative
spectrophotometry allowed to estimate the efficiency of Phe removal.
