摘要：The Leishmania major Parasite surface Antigen-2 (PSA-2) is a family of
glycoinositol phospholipids anchored glycoprotoins expressed in both
promastigotes and amastigotes. Promastigote PSA-2 comprises three polypeptides
with approximate molecular weight of 96, 80 and 50 kDa. Amastigote express a
distinct but closely PSA-2 polypeptide with molecular weight of 50 kDa. In this
study fusion of SP2/0 myeloma cells with immunized mice spleenocytes infected
with promastigotes of L. major intraperitoneally resulted to a clone of
hybridoma producing a specific antibody that only reacts with L. major parasite
surface antigen (PSA-2). This mAb showed no crossreactivity with either other
Leishmania species including L. tropica, L. donovani and L. infantum or
recombinant gp63. Western blot analysis of culture supernatant revealed multiple
bands with molecular weight of 50, 58, 80 and 96 kDa only in L. major.
关键词：Leishmania major ، Monoclonal antibody ، PSA-2
