期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2009
卷号：16
期号：01
页码：9-9
出版社：Institute of Agricultural Medicine in Lublin
摘要：In the wake of controversies surrounding the usefulness of PCR in the diagnostics
of borreliosis, the aim of the presented study was to monitor the presence of
B. burgdorferi s.l. in dogs with clinical borreliosis in the course of relevant treatment.
The monitoring was based on detecting borrelia’s DNA before- (study I), during- (study
II), and after completion of the therapy (study III). In addition, to rule out possible coinfections,
the dogs’ blood was examined for the presence of anaplasma, babesia and
rickettsia. Blood samples taken from 11 dogs, with clinically detected borreliosis, were
used for obtaining DNA for PCR. Positive results of PCR, with primers complementary
to the fl a gene, indicating the presence of DNA of B. burgdorferi s.l., were noted, in
study I, in the blood of 7 dogs (63.6% dogs), in study II in 3 dogs, while in study III all
blood samples were negative. In 6 out of 7 PCR+, the fi rst study was carried out during
week 1. Therefore, the PCR method is useful for monitoring early canine infections with
spirochetes B. burgdorferi s.l. In all positive samples, subjected to PCR-RFLP, it was the
case of a single genospecies, i.e. B. burgdorferi sensu stricto. Studies for the presence of
DNA of Babesia sp., as well as DNA of Ricettsia helvetica, were negative in all samples.
Anaplasma phagocytophilum DNA was detected in the blood of a single dog, and only
in study I. The same dog also proved positive for the presence of borrelia DNA. Co-occurrence
of both pathogens did not disturb the clinical picture of borreliosis, and the
administered treatment was also effective for the mixed infection.
