期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2009
卷号：16
期号：01
页码：143-143
出版社：Institute of Agricultural Medicine in Lublin
摘要：The objective of present work was to determine and compare the components
of bioaerosol in several sectors of plant processing industries. The study was conducted
in 10 facilities engaged in herb and grain processing, fl ax threshing, grain storing, baking,
and cereals production. The air samples were taken on glass fi bre fi lters with an AS-50
sampler. We determined the concentrations of airborne microorganisms, dust, endotoxin
and peptidoglycan. Total concentrations of viable airborne microorganisms ranged from
0.18–861.4 × 103 cfu/m3. The highest levels of microbial contamination of the air were
observed at fl ax farms, in grain elevators and in a herb processing plant. Gram-positive
bacteria and fungi were detected at all sampling sites and their median concentrations
were respectively 18.1 × 103 cfu/m3 and 0.66 × 103 cfu/m3. The concentration of Gramnegative
bacteria ranged from 0.0–168.0 × 103 cfu/m3. The concentration of thermophilic
actinomycetes ranged from 0.0–1.45 × 103 cfu/m3. Qualitatively, Gram-positive bacteria
constituted 23–93% of the total microbial count. The most common species were:
Staphylococcus spp., Curtobacterium pusillum, Rhodococcus fascians, Aureobacterium
testaceum, Sanguibacter keddieii, Microbacterium spp., and Bacillus spp. Gram-negative
bacteria formed 0–48% of the total count. The species Pantoea agglomerans dominated
in all examined air samples. Fungi constituted 2.5–76.9% of the total microbial count.
Among them, Penicillium spp., Mucor spp., Alternaria spp., Aspergillus niger, and Aspergillus
spp. were found. The dust concentration ranged from 0.18–86.9 mg/m3. The
concentration of endotoxin was large and ranged from 0.0041–1562.6 μg/m3. Muramic
acid, the chemical marker of peptidoglycan, was detected in 9 out of 13 (69.2%) collected
samples. The concentration of peptidoglycan ranged from 1.93–416 ng/m3. A highly signifi
cant correlation was found between the individual components of bioaerosol determined
in this study. The concentration of endotoxin was correlated with the concentration
of Gram-negative bacteria, total microorganisms, and peptidoglycan (R>0.9, p<0.001).
The concentration of peptidoglycan was correlated with the concentration of Gram-positive
bacteria, Gram-negative bacteria, and total microorganisms (R>0.9, p<0.001).
