标题：COINCIDENCE OF THRE PATHOGENS (BORRELIA BURGDORFERI SENSU LATO, ANAPLASMA PHAGOCYTOPHILUM AND BABESIA MICROTI) IN IXODES RICINUS TICKS IN THE LUBLIN MACROREGION
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2009
卷号：16
期号：01
页码：151-151
出版社：Institute of Agricultural Medicine in Lublin
摘要：Ticks are very important vectors of pathogenic microorganisms (viruses, bacteria,
protozoans), which may induce serious contagious diseases in humans and in farm
animals. The aim of the study was to determine the coincidence of 3 pathogens: Borrelia
burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes
ricinus ticks in south-eastern Poland and to estimate the degree of infection with each
of the examined pathogens depending on the developmental stage of ticks (nymph, female,
male). The study material were 1,620 Ixodes ricinus ticks collected at 5 sites in the
Lublin macroregion, showing the presence of various forest biotopes. The PCR method
was used to identify DNA for B. burgdorferi and A. phagocytophilum, and the nested-
PCR – for B. microti. In 1,368 (84.44%) of the 1,620 examined ticks no infections were
found. Single infections were noted in 217 ticks (13.4%) and coinfections were detected
in 35 specimens (2.16%). The most common was the coincidence of A. phagocytophilum
with B. microti (17 infected specimens, 1.05% of the total number). A similar result was
obtained for the coincidence of B. burgdorferi s. l. with A. phagocytophilum (15 infected
specimens, 0.93% of the total number). Only 2 cases of the coinfection of B. burgdorferi
s. l. with B. microti, which equals 0.12% of the total number, were found. Infection with
all 3 pathogens was identifi ed in only 1 female tick (0.06% of the total number).
