期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2009
卷号：16
期号：01
页码：173-173
出版社：Institute of Agricultural Medicine in Lublin
摘要：A novel inhalation challenge set for the study of experimental allergic alveolitis
(hypersensitivity pneumonitis) in mice was designed. A fi nely dispersed aerosol of allergenic
extract generated by the commercial ultrasonic nebulizer “TAJFUN MU1” (produced
by Medbryt, Warsaw, Poland) was transported to the airtight inhalation chamber.
In the chamber were placed 15 perforated containers made of transparent plastic, each
containing one mouse. They were coupled in 3 units, each consisted of 5 containers. The
constant fl ow of aerosol through the chamber was assured by commercial vacuum pump
“PL 2/3” (AGA LABOR S.C., Warsaw, Poland). The applied set enabled the natural exposure
of mice via the inhalation route to known quantities of allergen (usually microbial)
suspended in saline, and then dispersed in form of fi ne aerosol by ultrasonic nebulizer.
This method assures the penetration of allergen into the deep parts of lungs, alveoli and
bronchioli. The detailed study of histopathological and biochemical changes in the lungs
of exposed animals will be the subject of further publications. So far, the retention of endotoxin
in the lungs of mice exposed to the extract of a Gram-negative bacterium Pantoea
agglomerans and appearance of positive serologic reactions to this extract indicate the
effectiveness of the method.
