摘要：To assess the effects of chronical exposure to thiram on the female reproductive function by comparative approach of some epidemiological and experimental aspects. Material and methods: Five years epidemiological cohort study on 132 women occupationally exposed to thiram , in the synthesis industry, were compared to matched controls. Exposure evaluation by GC measurements of thiram in workplaces air and biotoxicological tests, too. Fertility questionnaires were used. The experiment studied thiram effects on fertility as well as genotoxic , embriotoxic and teratogenic potential of thiram in chronic experiments on Wistar female rats by using three doses. Results and discussion:High concentrations of thiram in the workplaces air, and important levels of its metabolite in urine were found. The epidemiological research has showed among the exposed women a significant decrease of the fertility, a higher frequency of spontaneous abortions (OR=3.52, 95% CI: 2.25-5.50), and a significantly increased risk of congenital malformations (OR=11.57, 95% CI: 2.13-66.86). The mutagenetic investigations indicated a significant increase in the number of chromosomal aberrations (p=0.004) and of micronuclei (p=0.01). In chronic experimental administration, the highest dose (1/3 LD50 ) determined a significant increase of the tardive resorbtion rate (p=0.004) and also a high proportion of major malformations (p=0.0001); the medium dose (1/9 LD50 ) and the low dose (1/27 LD50 ) generated a high rate of foetal loss. These results are confirmed by high rates of structural chromosomal aberrations and also micronucleus high rates. Conclusions : Epidemiological and experimental studies support the hypothesis that high exposure to thiram determines a significant fertility decrease and the qualitative impairment of offspring.
关键词： thiram , occupational exposure, experiments, reproductive function
