摘要：In the present study we evaluated the in vitro lymphocyte specific blastogenesis in response to stimulation with PPD (purified-proteine-derivate) in tuberculosis patients using the flow-cytometry method based on carbofluorescein diacetate succinimydil ester (CFSE) dye dilution technique. 14 individuals (7 PPD+ healthy donors and 7 tuberculosis patients) were studied. Mononuclear cells from peripheral blood or pleural fluid were initially stained with 1.25 μM CFSE, then they were cultivated with or without 20 μg/ml PPD for up to 7 days. At indicated time points the cells were collected, stained with anti-CD3 Cy Chrome and analyzed on a FACSCalibur flow cytometer. The results showed a poor reactivity of peripheral lymphocytes from tuberculosis patients as compared to that recorded in healthy, PPD-reactive persons, and to the values observed in the proliferative response of cells from pleural effusion.
