摘要：Salivary nitrate/nitrite concentration has often been used as a biomarker of human exposure to nitrate. A spectrophotometric method for estimation of nitrite in saliva is described. Nitrite (NO2-) is determined through formation of a reddish purple azo dye produced at pH 2.0 to 2.5 by coupling diazotized compound with 1-Naphthylamine. The method is suitable for the estimation of 0.25-10 m g/ml. The preservation procedure and deproteinization of saliva sample remove possible errors
关键词： nitrite in human saliva, spectrophotometric method
