摘要：In this study we examined the impact of carbon black particles (CBPs) type M120 on the reduced glutathione and proteins in the respiratory tract epithelial lining fluid (ELF) using a model exposure system. Model ELF solutions without (-) and with (+) chelating agents were incubated with 500 m g/mL of CBPs in 1 mL aliquots (37oC) for 0-6h under mixed conditions. At set intervals throughout the exposure, CBPs were removed by centrifugation, and samples rapidly frozen prior to reduced glutathione (GSH), proteins and protein thiols determination. It has been demonstrated that both albumin and chelating agents can protect oxidation of reduced glutathione induced by carbon black particles.
