NMR spectroscopy is one of the most important techniques used in the structural identification of a compound. For this reason, a spectroscopy course that includes NMR is required for undergraduate chemistry students. However, many students have difficulty understanding the concepts of NMR spectroscopy and how to interpret NMR spectra. An interactive NMR (iNMR) course containing highly interactive animations and exercises was developed based on constructivist learning environments to support self-studying and to provide practice in NMR problem solving. Third-year chemistry students (n = 26) volunteered to use the course as a supplement to their regular spectroscopy class. Students’ understanding of key NMR spectroscopy concepts was statistically increased after completing the interactive course. Moreover, the average course grade of students completing the iNMR course was significantly higher than that of students completing traditional lessons in each of the previous four years.