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  • 标题:Screening, Identification of best Producers and Optimization of Extracellular Proteases from Moderately Halophilic Alkalithermotolerant Indigenous Actinomycetes
  • 本地全文:下载
  • 作者:Debananda S. Ningthoujam ; Pintubala Kshetri ; Suchitra Sanasam
  • 期刊名称:World Applied Sciences Journal
  • 印刷版ISSN:1818-4952
  • 电子版ISSN:1991-6426
  • 出版年度:2010
  • 卷号:7
  • 期号:07
  • 出版社:International Digital Organization for Scientific Information Publications
  • 摘要:

    Of 14 actinomycete isolates in our collection from various biotopes in Manipur that were screened
    on skim milk agar, 5 strains produced high amounts of extracellular proteases as indicated by large halos. One
    isolate HA-4, obtained from limestone quarry sample, was selected for further studies. The selected isolate was
    subjected to polyphasic characterization and identified as Nocardiopsis prasina HA-4. The organism grew well
    at temperatures of 20-42°C, pH 7-10 and in presence of 2-10% NaCl (with more growth at 10% salt). Cultural and
    environmental conditions for protease production by HA-4 were studied. Sucrose (5%,w/v) and peptone (0.2%,
    w/v) were found to be the best C and N sources for protease production respectively. The optimum temperature
    for the enzyme from HA-4 was 55°C and it had two pH optima at 7.0 and 10.0. Ca2+ and Mg2+ partially inhibited
    enzyme activity whereas Hg2+ completely inhibited it. Interestingly Fe2+ was found to have stimulatory effect,
    which has been rarely reported. The wide tolerance of temperature and pH by Nocardiopsis prasina HA-4
    protease makes it an ideal candidate to be investigated further for potential application as a detergent additive.

  • 关键词:Actinomycetes ; Alkalithermotolerant ; HA-4 ; Manipur ; Nocardiopsis prasina ; Protease
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