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  • 标题:Comparação entre diferentes descalcificadores no estudo da ultraestrutura dentária em peixes
  • 本地全文:下载
  • 作者:Alexandre Scharcansky ; Carlos Alberto Santos de Lucena
  • 期刊名称:Biociências
  • 印刷版ISSN:0104-3455
  • 出版年度:2008
  • 卷号:16
  • 期号:2
  • 出版社:Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul
  • 摘要:Three types of agent to etching teeth were tested to verify which presents better performance in the visualization in SEM of the apatite crystals - mineral deposited in the skeleton of the vertebrates, including teeth - that compose the dental enameloid tissue: EDTA, hydrochloric acid, and phosphoric acid. The best solution was through the immersion of the teeth in phosphoric acid 15% from 4 to 15 seconds, depending on the size of the teeth. After decalcification the teeth were submitted to the protocol commonly used in preparing the piece for observation in SEM.
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